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eLABa objektas: "Žieminių ir vasarinių rapsų (Brassica napus L.) genetinės įvairovės įvertinimas naudojant AAPD analizę", 2010,D:20100614:095323-49989 |
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Tubelytė, Vaida - Magistro baigiamojo darbo vertinimo posėdžio sekretorius
Radzijevskaja, Jana - Magistro baigiamojo darbo recenzentas
Rugienius, Rytis - Magistro baigiamojo darbo vertinimo komisijos pirmininkas
Paulauskas, Algimantas - Magistro baigiamojo darbo vadovas
Vytauto Didžiojo universitetas - Mokslinį laipsnį teikianti institucija
Genetic polymorphism of spring and winter oilseed rape (Brassica napus L.) using RAPD analysis
Oilseed rape (Brassica napus L.) is an important oil crop grown in many countries and also in Lithuania and supplying oil, feed, bio-fuel, etc. As far as a notification was presented about GM spring rape (Brassica napus L.) field trials (BASF Plant Science GmbH) in 2006, it is important to detect the zero level of gene flow within and between oilseed rape populations and individual plants, with other Cruciferae and feral species. Winter and spring rape cultivars were gathered from different region – for 10 to 30 individuals each. RAPD analysis was used in the study. Twelve primers were screened for their ability to produce polymorphic patterns: OPA-01, OPA-04, OPA-09, OPA-11, 222, 250, 268, 269, 340, 474, 516, 563. Dendrograms based on UPGMA cluster analysis confirmed the suitability of all the primers for the further analysis.RAPD data showed a significant genetic variation among oilseed rape of various cultivars and also inside cultivars. |
Raktažodžiai: RAPD, genetic polymorphism, oilseed rape, Brassica napus L. |